Myonuclei (Cherry NLS, red) and sarcomeres (Sls-GFP, green) dynamics in Nesprin/klar mutant muscle.
Representative movie of nuclei dynamics in Nesprin/klar mutant muscle.
Note how the nuclei and their nucleolei deform during contraction.
Also note how the marked nucleus fall behind during muscle relaxation.
Dynamics of microtubules (alpha-tubulin-GFP ,green), nucleus and the nucleolus (both marked with Cherry-NLS, red), during muscle contraction.
Ca2+ (Gcamp-GFP) temporal dynamics coupled to muscle contraction and relaxation (z-lines marked with zasp-RFP)
3D view of salivary gland chromatin
(Histone2B-mRFP, red) with two insertions
of multiple repeats of LacO sequences
labeled with lacI-GFP (green).
Spatial organization of RNA polymerase II (Rpb3-GFP, green), and chromatin (Histone2B-mRFP, red) as found in a cross section through a live myonuclous. Note the organization of POL II at the circumference of chromatin clusters.
Additional images obtained using the device were featured on Biomedical Picture of the Day,
managed by the MRC London Institute of Medical Sciences - Click the images
The minimal constraint device for imaging nuclei in live intact Drosophila larvae allows visualization and quantification of dynamic processes such as muscle contraction, nuclear deformations, and chromatin organization in their intrinsic environment.
Assembling the device and placing it on the microscope stage, while the larva is facing the lens.